Eutrophication is one of the most relevant man‐induced changes occurring in coastal waters. The identification and assessment of specific responses to eutrophication in seagrasses can provide a useful tool for the detection of changes in the water quality in coastal zones, given the wide range of distribution of these organisms. In this study, we combine a correlational (across‐sites comparison) and a manipulative (fertilization experiment) approach to evaluate the usefulness and potential of alkaline phosphatase activity (APA) in the endemic Mediterranean seagrass Posidonia oceanica as an eutrophication biomarker. Our results showed that APA decreases promptly following nutrient additions, the response being maintained except during the winter period. APA also varies across natural meadows under different levels of nutrient discharges at scales relevant for monitoring purposes. AP activity seems to be an optimal ‘physiological biomarker’ that responds promptly and reliably to a pulse of eutrophication exposure. However, other considerations, such as the seasonality (the response disappears in winter), suggest its use with some caution and, as far as possible, as a complement of other bio‐indicators. 相似文献
One-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and high resolution two-dimensional electrophoresis (2-DE) were applied to separate protein molecules in dissolved organic matter (DOM) from oceanic waters. Results were: (1) The 2-DE distinguished a total of 412 protein spots in 10 samples from five water columns over the Pacific, although fewer than 30 proteins were resolved as bands from the identical samples by SDS-PAGE. (2) Major and ubiquitous protein bands (34 and 39 kDa proteins) on the SDS-PAGE gel were resolved into horizontally spread arrays (trains) of spots on the 2-DE gels, indicating that these bands were a mixture of protein species that have the same molecular weight (MW) but different isoelectric points (pIs). (3) Proteins that exhibited such electrophoretic patterns on the 2-DE gels were glycosylated with variable linkages between the sugar and polypeptide chains. (4) N-terminal amino-acid sequencing demonstrated that individual spots within each train of spots had identical N-terminal amino-acid sequences.The N-terminal amino-acid sequences of the 39 and 34 kDa glycoprotein spots in samples collected at different sites were also identical. Protein isoforms with the same amino-acid sequence but different glycosylation profiles, termed glycoforms, were often observed on the 2-DE gel. Thirty-one and 24 spots on the 2-DE gels were glycoforms of two glycoproteins with MWs of 39 and 34 kDa, respectively; they were one protein species. The glycoforms of the 39 kDa protein were identified as a low molecular weight alkaline phosphatase (L-AP) of Pseudomonas aeruginosa PAO1 by a homology search through five amino-acid sequence databases. The present and earlier work indicates that all identified source organisms of dissolved proteins belong to the Pseudomonas group. We propose the hypothesis that proteins associated with membrane vesicles liberated from a minor member of the bacterioplankton assemblage, the marine Pseudomonas group, are one of the important sources of dissolved proteins in oceanic waters. This hypothesis may apply to the source pathway and survival not only of proteins and also to the universally occurring bacterial peptidoglycan and lipopolysaccharide components in DOM. 相似文献
In situ Sr-isotope and microchemical studies were used to determine the provenance of K-feldspar megacrysts hosted in mafic alkaline potassic, ultrapotassic rocks and in differentiated rocks from two nearby volcanic apparatus in central Italy.
At Monte Cimino volcanic complex, mafic leucite-free ultrapotassic megacryst-bearing rocks of olivine latitic composition are associated with evolved latite and trachyte. Here, latites and trachytes straddle the sub-alkaline field. Age-corrected 87Sr/86Sr values (Sri) of the analysed Cimino olivine latites vary from 0.71330 and 0.71578 and strongly increase at constant Mg value. Latite and trachyte have lower Sri than olivine latites ranging between 0.71331 and 0.71361. Sri of K-feldspar megacrysts from olivine latites are between 0.71352 and 0.71397, but core and rim 87Sr/86Sr ratios within individual megacryst are indistinguishable. In all the mafic rocks, the megacrysts are not in isotopic equilibrium with the hosts. K-feldspar megacrysts from both the latite and trachyte have similar Sr-isotope compositions (Sri=0.71357–0.71401) to those in the olivine latites. However, Sri of megacryst in the trachyte vary significantly from core to rim (Sri from 0.71401 to 0.71383). As with the olivine latites, the K-feldspar megacrysts are not in isotopic equilibrium with bulk rock compositions of the latite or trachyte.
At Vico volcano, megacryst-bearing rocks are mafic leucite-free potassic rocks, mafic leucite-bearing ultrapotassic rocks and old trachytic rocks. The mafic leucite-bearing and leucite-free rocks are a tephri-phonolite and an olivine latite, respectively. A megacryst in Vico trachyte is isotopically homogeneous (Sri CORE=0.71129, RIM=0.71128) and in equilibrium with the host rock (Sri bulk ROCK=0.71125). Sri of megacryst from tephri-phonolite is clearly not in isotopic equilibrium with its host (Sri bulk ROCK=0.71158), and it increases from core (Sri=0.71063) to rim (Sri=0.71077). A megacryst in Vico olivine latite is isotopically homogeneous (Sri CORE=0.71066, RIM=0.71065), but not in equilibrium with the host rock (Sri bulk ROCK=0.71013).
The Sr isotope microdrilling technique reveals that Cimino megacrysts were crystallised in a Cimino trachytic magma and were subsequently incorporated by mixing/mingling processes in the latitic and olivine latitic melts. A model invoking the presence of a mafic sub-alkaline magma, which was mixed with the olivine latite, is proposed to justify the lack of simple geochemical mixing relation between Cimino trachytes and olivine latites. This magmatological model is able to explain the geochemical characteristics of Cimino olivine latites, otherwise ascribed to mantle heterogeneity.
The similarity of core Sri of megacrysts hosted in Vico tephri-phonolite and olivine latite suggests that the K-feldspar megacrysts are co-genetic. Isotopic equilibrium between megacryst and Vico host trachyte indicates that the trachyte is the parent of this megacryst. On the contrary, the megacrysts hosted in tephri-phonolite and olivine latite do not derive from the old trachytic magma because no diffusion process may explain the core to rim Sr isotope increase of the xenocryst hosted in the tephri-phonolite. The megacrysts hosted in the Vico mafic rocks might derive from a trachytic melt similar in composition to the old Vico trachytes. 相似文献
The intrusive complex at Hortavær represents a magma transfer zone in which multiple pulses of gabbroic and dioritic magmas evolved along Fe- and alkali-enrichment trends. Extreme alkali enrichment resulted in nepheline-normative and sparse nepheline-bearing monzodioritic and monzonitic rocks. More evolved monzonitic and syenitic rocks are silica saturated and, in some cases, quartz bearing. Previous and current research recognized an abundance of clinopyroxene and other Ca-rich phases, such as scapolite, grossular-rich garnet, and igneous-textured calcite among the mafic and intermediate rocks. Even the most pyroxene-rich samples contain low Sc concentrations, which suggests early, intense fractionation of clinopyroxene. These features and the alkali enrichment are consistent with assimilation of carbonate-rich host rocks. Carbon isotope ratios of the igneous-textured calcite indicate an origin of the carbon from host rocks rich in calcite, consistent with assimilation. However, low Nd values (−3.4 to −10.2) and moderate initial 87Sr/86Sr values (0.7052 to 0.7099) indicate the need for assimilation of quartzofeldspathic rocks as well. Models of combined assimilation and fractional crystallization indicate that assimilation of simple end members, either carbonate or silicate, cannot explain the entire data set. Instead, variable proportions of carbonate and silicate materials were assimilated, with the most pronounced assimilation effects in the mafic rocks. The reasons for variable degrees of assimilation are, as yet, uncertain. It is possible that assimilation of calc-silicate rocks with variable carbonate/silicate proportions resulted in the range of observed compositions. However, the importance of carbonate assimilation in mafic rocks compared to felsic ones suggests that assimilation of carbonates was predominant at high temperature and/or mafic magma compositions and assimilation of silicates was predominant at lower temperature and/or felsic magma compositions. We suggest that the ability of the mafic magma to dissolve higher proportions of carbonate contaminants is the result of the magma's ability to form clinopyroxene as a product of assimilation. In any case, extensive carbonate assimilation was possible because CO2 escaped from the system. 相似文献
